Welcome to the PhosphoPICK Server
PhosphoPICK is a method for predicting kinase substrates using an integrated system of cellular context and protein sequence information, and is currently able to make predictions for 107 human kinases, 24 mouse kinases and 26 yeast kinases. If this is your first time using PhosphoPICK, the tutorial section contains more information on how the algorithm works, and how to interpret the results.
There are three ways to use PhosphoPICK:
- The Submit Sequences page allows you to submit protein sequences, choose one or more kinases, and obtain predictions for the potential phosphorylation sites in your protein sequences.
- The Download page is for those interested in downloading proteome-wide predictions of kinase substrates.
- The SNV analysis page is for the analysis of the effect of mutations on potential phosphorylation sites in protein sequences.
If you find PhosphoPICK useful in your research please cite one of the following papers:
- Patrick R, Horin C, Kobe B, Le Cao KA and Bodén M (2016) Prediction of kinase-specific phosphorylation sites through an integrative model of protein context and sequence. BBA - Proteins and Proteomics. 1864(11):1599-608. DOI: 10.1016/j.bbapap.2016.08.001. [Pubmed Abstract].
- Patrick R, Kobe B, Le Cao KA and Bodén M (2017) PhosphoPICK-SNP: Quantifying the effect of amino acid variants on protein phosphory lation, Bioinformatics. DOI: 10.1093/bioinformatics/btx072. [PubMed Abstract].
- Patrick R, Le Cao KA, Kobe B and Bodén M (2015) PhosphoPICK: Modelling cellular context to map kinase-substrate phosphorylation eve nts. Bioinformatics. 31(3):382-389. DOI: 10.1093/bioinformatics/btu663. [Pubmed Abstract].